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murine dlbcl cell line a20  (ATCC)


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    ATCC murine dlbcl cell line a20
    Murine Dlbcl Cell Line A20, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1364 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/murine+dlbcl+cell/A20/pm39323416-215-28-50
    Average 97 stars, based on 1364 article reviews
    murine dlbcl cell line a20 - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Microarray:

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint
    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).

    Quantitative RT-PCR:

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint
    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).

    Expressing:

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint
    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).

    Knockdown:

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint
    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).

    CCK-8 Assay:

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint
    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).

    Transwell Invasion Assay:

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint
    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).

    Western Blot:

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint
    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).

    Staining:

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint
    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).

    Luciferase:

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint
    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).

    Reporter Assay:

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint
    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).

    Over Expression:

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint
    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).

    Transfection:

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint
    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).

    Binding Assay:

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint
    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).. GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).GM12878 cells were grown in RPMI 1640 (HyClone, Logan, UT, USA) with GlutaMAX Supplement (Thermo Fisher Scientific, Waltham, MA, USA), 15% fetal bovine serum (FBS; Sigma-Aldrich, St. Louis, MO, USA), and 1% pen/strep (Invitrogen, Carlsbad, CA, USA).



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    murine dlbcl cell line a20  (ATCC)
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    a Hierarchical clustering showed the differentially expressed lncRNAs in <t>DLBCL</t> tissues compared with the paired para-tumor tissues according to the microarray analysis (Fold change > 2, P < 0.05). b The expressions of top-5 upregulated lncRNAs in DLBCL tissues in TCGA DLBCL samples were analyzed through GEPIA. c RT-qPCR data showed the upregulated expression of SNHG14 in DLBCL cell lines. d Knockdown of SNHG14 in FARAGE <t>and</t> <t>U2932</t> cells was confirmed by RT-qPCR. e – f Viability and colony generation of DLBCL cells were evaluated by CCK-8 and colony formation assays. g Invasion of DLBCL cells was detected by transwell invasion assay. Scale bar: 100 μm. h – i EMT markers (E-cadherin and N-cadherin) were detected by western blot and IF staining assay in DLBCL cells. Scale bar: 50 μm. * P < 0.05, ** P < 0.01
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    a20 murine diffuse large b cell lymphoma dlbcl cells  (ATCC)
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    Figure 1. Effects of glucose on the expression of HMGA2 and markers of epithelial-mesenchymal transition (EMT) in <t>A20</t> murine diffuse large B-cell lymphoma <t>(DLBCL)</t> cells. A20 cells in RPMI-1640 medium and 10% fetal bovine serum (FBS) were treated with different concentrations of glucose, including normal glucose (NG), 5mM, high glucose-1 (HG-1) 25mM, and high glucose-2 (HG-2), 30 mM. Gene expression was analyzed 48 hours later by quantitative real-time polymerase chain reaction (qRT-PCR) for HMGA2 (A) and EMT markers including E-cadherin, N-cadherin, snail, and slug (C). The protein levels of HMGA2 (B) and EMT markers (D) in A20 cells treated with high glucose (30 mM) and normal glucose (5 mM) were assessed by Western blot. Quantification analysis by ImageJ software was performed, as shown as a graph on the right. GAPDH was used as an internal reference. Each experiment was performed in triplicate, and the representative images are shown. Data are presented as the mean ± standard deviation (SD). * P<0.05, compared with the Control (normal glucose) group.
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    Image Search Results


    a Hierarchical clustering showed the differentially expressed lncRNAs in DLBCL tissues compared with the paired para-tumor tissues according to the microarray analysis (Fold change > 2, P < 0.05). b The expressions of top-5 upregulated lncRNAs in DLBCL tissues in TCGA DLBCL samples were analyzed through GEPIA. c RT-qPCR data showed the upregulated expression of SNHG14 in DLBCL cell lines. d Knockdown of SNHG14 in FARAGE and U2932 cells was confirmed by RT-qPCR. e – f Viability and colony generation of DLBCL cells were evaluated by CCK-8 and colony formation assays. g Invasion of DLBCL cells was detected by transwell invasion assay. Scale bar: 100 μm. h – i EMT markers (E-cadherin and N-cadherin) were detected by western blot and IF staining assay in DLBCL cells. Scale bar: 50 μm. * P < 0.05, ** P < 0.01

    Journal: Cell Death & Disease

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint

    doi: 10.1038/s41419-019-1886-5

    Figure Lengend Snippet: a Hierarchical clustering showed the differentially expressed lncRNAs in DLBCL tissues compared with the paired para-tumor tissues according to the microarray analysis (Fold change > 2, P < 0.05). b The expressions of top-5 upregulated lncRNAs in DLBCL tissues in TCGA DLBCL samples were analyzed through GEPIA. c RT-qPCR data showed the upregulated expression of SNHG14 in DLBCL cell lines. d Knockdown of SNHG14 in FARAGE and U2932 cells was confirmed by RT-qPCR. e – f Viability and colony generation of DLBCL cells were evaluated by CCK-8 and colony formation assays. g Invasion of DLBCL cells was detected by transwell invasion assay. Scale bar: 100 μm. h – i EMT markers (E-cadherin and N-cadherin) were detected by western blot and IF staining assay in DLBCL cells. Scale bar: 50 μm. * P < 0.05, ** P < 0.01

    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).

    Techniques: Microarray, Quantitative RT-PCR, Expressing, Knockdown, CCK-8 Assay, Transwell Invasion Assay, Western Blot, Staining

    a Heat map showed the expressions of 124 miRNAs potentially targeted by SNHG14 in 3 DLBCL tissues compared with the matched non-tumor tissues. b Luciferase reporter assay was conducted to detect the interaction between the top 5 downregulated miRNAs with SNHG14. c RT-qPCR analysis of miR-5590-3p expression in DLBCL cell lines and normal cell line. d RT-qPCR analysis following the RIP assay was conducted to confirm the interaction between miR-5590-3p with SNHG14 in DLBCL cells. e Interacting sequences on SNHG14 for miR-5590-3p were obtained from Starbase3.0 and mutated by altering them with the complementary sequences. Luciferase reporter assay was performed to detect the interaction between SNHG14 with miR-5590-3p. f Pearson’s correlation curve showed the negative relation between SNHG14 and miR-5590-3p in DLBCL tissues. g Overexpression of miR-5590-3p in DLBCL cells was confirmed by RT-qPCR assay. h Expression of SNHG14 upon miR-5590-3p overexpression in DLBCL cells was detected by RT-qPCR. i Expression of miR-5590-3p upon SNHG14 silence in DLBCL was detected by RT-qPCR. * P < 0.05, ** P < 0.01

    Journal: Cell Death & Disease

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint

    doi: 10.1038/s41419-019-1886-5

    Figure Lengend Snippet: a Heat map showed the expressions of 124 miRNAs potentially targeted by SNHG14 in 3 DLBCL tissues compared with the matched non-tumor tissues. b Luciferase reporter assay was conducted to detect the interaction between the top 5 downregulated miRNAs with SNHG14. c RT-qPCR analysis of miR-5590-3p expression in DLBCL cell lines and normal cell line. d RT-qPCR analysis following the RIP assay was conducted to confirm the interaction between miR-5590-3p with SNHG14 in DLBCL cells. e Interacting sequences on SNHG14 for miR-5590-3p were obtained from Starbase3.0 and mutated by altering them with the complementary sequences. Luciferase reporter assay was performed to detect the interaction between SNHG14 with miR-5590-3p. f Pearson’s correlation curve showed the negative relation between SNHG14 and miR-5590-3p in DLBCL tissues. g Overexpression of miR-5590-3p in DLBCL cells was confirmed by RT-qPCR assay. h Expression of SNHG14 upon miR-5590-3p overexpression in DLBCL cells was detected by RT-qPCR. i Expression of miR-5590-3p upon SNHG14 silence in DLBCL was detected by RT-qPCR. * P < 0.05, ** P < 0.01

    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).

    Techniques: Luciferase, Reporter Assay, Quantitative RT-PCR, Expressing, Over Expression

    a Expression of ZEB1 in DLBCL tissues versus paired para-tumorous tissues was detected by RT-qPCR. b Expression of ZEB1 in DLBCL cell lines and normal cell line was detected by RT-qPCR. c Pearson’s correlation curve showed that ZEB1 was positively correlated with SNHG14 and negatively correlated with miR-5590-3p in DLBCL tissues. d Interacting sequences on ZEB1 for miR-5590-3p were obtained from Starbase3.0 and mutated by altering them with the complementary sequences. Luciferase reporter assay was performed to detect the interaction between ZEB1 with miR-5590-3p. e RT-qPCR analysis following the RIP assay was conducted to confirm the interaction between miR-5590-3p with ZEB1 and SNHG14 in DLBCL cells. f Expressions of ZEB1 mRNA and protein and PD-L1 protein upon miR-5590-3p overexpression in DLBCL cells were detected by RT-qPCR and western blot. g Expressions of ZEB1 mRNA and protein and PD-L1 protein upon indicated transfection in DLBCL cells were detected by RT-qPCR and western blot. * P < 0.05, ** P < 0.01

    Journal: Cell Death & Disease

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint

    doi: 10.1038/s41419-019-1886-5

    Figure Lengend Snippet: a Expression of ZEB1 in DLBCL tissues versus paired para-tumorous tissues was detected by RT-qPCR. b Expression of ZEB1 in DLBCL cell lines and normal cell line was detected by RT-qPCR. c Pearson’s correlation curve showed that ZEB1 was positively correlated with SNHG14 and negatively correlated with miR-5590-3p in DLBCL tissues. d Interacting sequences on ZEB1 for miR-5590-3p were obtained from Starbase3.0 and mutated by altering them with the complementary sequences. Luciferase reporter assay was performed to detect the interaction between ZEB1 with miR-5590-3p. e RT-qPCR analysis following the RIP assay was conducted to confirm the interaction between miR-5590-3p with ZEB1 and SNHG14 in DLBCL cells. f Expressions of ZEB1 mRNA and protein and PD-L1 protein upon miR-5590-3p overexpression in DLBCL cells were detected by RT-qPCR and western blot. g Expressions of ZEB1 mRNA and protein and PD-L1 protein upon indicated transfection in DLBCL cells were detected by RT-qPCR and western blot. * P < 0.05, ** P < 0.01

    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).

    Techniques: Expressing, Quantitative RT-PCR, Luciferase, Reporter Assay, Over Expression, Western Blot, Transfection

    a DNA motif of ZEB1 and the predicted binding sites on PD-L1 promoter were obtained from JASPAR. b Silence of ZEB1 in FARAGE and U2932 cells was confirmed by RT-qPCR. c Expressions of PD-L1 mRNA and protein and ZEB1 protein upon ZEB1 silence in DLBCL cells were detected by RT-qPCR and western blot. d – e ChIP and luciferase reporter assays showed that ZEB1 bound to both sites 1 and 2 on PD-L1 promoter. f The predicted ZEB1 binding sites on SNHG14 promoter were obtained from JASPAR. g – h ChIP and luciferase reporter assays showed that ZEB1 bound to both sites 1 and 2, rather than site 3 on SNHG14 promoter. i Expression of SNHG14 upon ZEB1 silence in DLBCL cells was detected by RT-qPCR. * P < 0.05, ** P < 0.01

    Journal: Cell Death & Disease

    Article Title: LncRNA SNHG14/miR-5590-3p/ZEB1 positive feedback loop promoted diffuse large B cell lymphoma progression and immune evasion through regulating PD-1/PD-L1 checkpoint

    doi: 10.1038/s41419-019-1886-5

    Figure Lengend Snippet: a DNA motif of ZEB1 and the predicted binding sites on PD-L1 promoter were obtained from JASPAR. b Silence of ZEB1 in FARAGE and U2932 cells was confirmed by RT-qPCR. c Expressions of PD-L1 mRNA and protein and ZEB1 protein upon ZEB1 silence in DLBCL cells were detected by RT-qPCR and western blot. d – e ChIP and luciferase reporter assays showed that ZEB1 bound to both sites 1 and 2 on PD-L1 promoter. f The predicted ZEB1 binding sites on SNHG14 promoter were obtained from JASPAR. g – h ChIP and luciferase reporter assays showed that ZEB1 bound to both sites 1 and 2, rather than site 3 on SNHG14 promoter. i Expression of SNHG14 upon ZEB1 silence in DLBCL cells was detected by RT-qPCR. * P < 0.05, ** P < 0.01

    Article Snippet: Human lymphoblastoid B cell (GM12878), human renal epithelial cell (293T), murine DLBCL cell (A20), and human DLBCL cells (OCI-LY7, DB, U2932, and FARAGE) were purchased from American Type Culture Collection (ATCC; Manassas, VA, USA).

    Techniques: Binding Assay, Quantitative RT-PCR, Western Blot, Luciferase, Expressing

    Figure 1. Effects of glucose on the expression of HMGA2 and markers of epithelial-mesenchymal transition (EMT) in A20 murine diffuse large B-cell lymphoma (DLBCL) cells. A20 cells in RPMI-1640 medium and 10% fetal bovine serum (FBS) were treated with different concentrations of glucose, including normal glucose (NG), 5mM, high glucose-1 (HG-1) 25mM, and high glucose-2 (HG-2), 30 mM. Gene expression was analyzed 48 hours later by quantitative real-time polymerase chain reaction (qRT-PCR) for HMGA2 (A) and EMT markers including E-cadherin, N-cadherin, snail, and slug (C). The protein levels of HMGA2 (B) and EMT markers (D) in A20 cells treated with high glucose (30 mM) and normal glucose (5 mM) were assessed by Western blot. Quantification analysis by ImageJ software was performed, as shown as a graph on the right. GAPDH was used as an internal reference. Each experiment was performed in triplicate, and the representative images are shown. Data are presented as the mean ± standard deviation (SD). * P<0.05, compared with the Control (normal glucose) group.

    Journal: Medical Science Monitor

    Article Title: High Glucose Promotes Epithelial-Mesenchymal Transition, Migration and Invasion in A20 Murine Diffuse Large B-Cell Lymphoma Cells Through Increased Expression of High Mobility Group AT-Hook 2 (HMGA2)

    doi: 10.12659/msm.916195

    Figure Lengend Snippet: Figure 1. Effects of glucose on the expression of HMGA2 and markers of epithelial-mesenchymal transition (EMT) in A20 murine diffuse large B-cell lymphoma (DLBCL) cells. A20 cells in RPMI-1640 medium and 10% fetal bovine serum (FBS) were treated with different concentrations of glucose, including normal glucose (NG), 5mM, high glucose-1 (HG-1) 25mM, and high glucose-2 (HG-2), 30 mM. Gene expression was analyzed 48 hours later by quantitative real-time polymerase chain reaction (qRT-PCR) for HMGA2 (A) and EMT markers including E-cadherin, N-cadherin, snail, and slug (C). The protein levels of HMGA2 (B) and EMT markers (D) in A20 cells treated with high glucose (30 mM) and normal glucose (5 mM) were assessed by Western blot. Quantification analysis by ImageJ software was performed, as shown as a graph on the right. GAPDH was used as an internal reference. Each experiment was performed in triplicate, and the representative images are shown. Data are presented as the mean ± standard deviation (SD). * P<0.05, compared with the Control (normal glucose) group.

    Article Snippet: A20 murine diffuse large B-cell lymphoma (DLBCL) cells (ATCC, Manassas, VA, USA) were routinely cultured in complete RPMI1640 medium (Gibco, Thermofisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS), and maintained at 37°C in a humidified atmosphere containing 5% CO2.

    Techniques: Expressing, Gene Expression, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Software, Standard Deviation, Control

    Figure 2. Effects of glucose on migration and invasion of A20 murine diffuse large B-cell lymphoma (DLBCL) cells. A20 cells were treated with different concentrations of glucose, including normal glucose (NG), 5 mM, high glucose (HG), 30 mM. Cell migration (A) and invasion (B) of A20 cells were evaluated by transwell assays. Quantification analysis showed that migration and invasion in A20 cells treated with high glucose were enhanced compared with the normal glucose group. Data are presented as the mean ± standard deviation (SD). * P<0.05, compared with the Control (normal glucose) group.

    Journal: Medical Science Monitor

    Article Title: High Glucose Promotes Epithelial-Mesenchymal Transition, Migration and Invasion in A20 Murine Diffuse Large B-Cell Lymphoma Cells Through Increased Expression of High Mobility Group AT-Hook 2 (HMGA2)

    doi: 10.12659/msm.916195

    Figure Lengend Snippet: Figure 2. Effects of glucose on migration and invasion of A20 murine diffuse large B-cell lymphoma (DLBCL) cells. A20 cells were treated with different concentrations of glucose, including normal glucose (NG), 5 mM, high glucose (HG), 30 mM. Cell migration (A) and invasion (B) of A20 cells were evaluated by transwell assays. Quantification analysis showed that migration and invasion in A20 cells treated with high glucose were enhanced compared with the normal glucose group. Data are presented as the mean ± standard deviation (SD). * P<0.05, compared with the Control (normal glucose) group.

    Article Snippet: A20 murine diffuse large B-cell lymphoma (DLBCL) cells (ATCC, Manassas, VA, USA) were routinely cultured in complete RPMI1640 medium (Gibco, Thermofisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS), and maintained at 37°C in a humidified atmosphere containing 5% CO2.

    Techniques: Migration, Standard Deviation, Control

    Figure 4. Effects of knockdown of the HMGA2 gene on the expression of protein markers of epithelial-mesenchymal transition (EMT) and HMGA2 in A20 murine diffuse large B-cell lymphoma (DLBCL) cells. A20 cells were transfected with siHMGA2 (50 nM), and then treated with or without high glucose (30 mM) for 48 hours. The protein levels of HMGA2 (A) and EMT markers (B) were assessed by Western blot separately, and quantification analysis was performed and shown as a graph right next to the images. GAPDH was used as an internal reference. Each experiment was performed in triplicate, and the representative images are shown. Data are presented as the mean ± standard deviation (SD). * P<0.05, compared with NC group; # P<0.05, compared with NC+HG group.

    Journal: Medical Science Monitor

    Article Title: High Glucose Promotes Epithelial-Mesenchymal Transition, Migration and Invasion in A20 Murine Diffuse Large B-Cell Lymphoma Cells Through Increased Expression of High Mobility Group AT-Hook 2 (HMGA2)

    doi: 10.12659/msm.916195

    Figure Lengend Snippet: Figure 4. Effects of knockdown of the HMGA2 gene on the expression of protein markers of epithelial-mesenchymal transition (EMT) and HMGA2 in A20 murine diffuse large B-cell lymphoma (DLBCL) cells. A20 cells were transfected with siHMGA2 (50 nM), and then treated with or without high glucose (30 mM) for 48 hours. The protein levels of HMGA2 (A) and EMT markers (B) were assessed by Western blot separately, and quantification analysis was performed and shown as a graph right next to the images. GAPDH was used as an internal reference. Each experiment was performed in triplicate, and the representative images are shown. Data are presented as the mean ± standard deviation (SD). * P<0.05, compared with NC group; # P<0.05, compared with NC+HG group.

    Article Snippet: A20 murine diffuse large B-cell lymphoma (DLBCL) cells (ATCC, Manassas, VA, USA) were routinely cultured in complete RPMI1640 medium (Gibco, Thermofisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS), and maintained at 37°C in a humidified atmosphere containing 5% CO2.

    Techniques: Knockdown, Expressing, Transfection, Western Blot, Standard Deviation

    Figure 5. HMGA2 expression was involved in epithelial-mesenchymal transition (EMT) induced by high glucose in A20 murine diffuse large B-cell lymphoma (DLBCL) cells by regulating the Wnt/b-catenin signaling pathway. A20 cells were transfected with siHMGA2 (50 nM), and then treated with high glucose (30 mM) for 48 hours. Western blot was performed to explore the effects of HMGA2 knockdown on the Wnt/b-catenin signaling pathway. The expression of key proteins including b-catenin, cyclin D1 and c-myc in the signaling pathway was assessed, and quantification analysis was performed and shown as a graph right next to the images. GAPDH was used as an internal reference. Each experiment was performed in triplicate and representative images are shown. Data are presented as the mean ± standard deviation (SD). * P<0.05, compared with the Control (normal glucose) group.

    Journal: Medical Science Monitor

    Article Title: High Glucose Promotes Epithelial-Mesenchymal Transition, Migration and Invasion in A20 Murine Diffuse Large B-Cell Lymphoma Cells Through Increased Expression of High Mobility Group AT-Hook 2 (HMGA2)

    doi: 10.12659/msm.916195

    Figure Lengend Snippet: Figure 5. HMGA2 expression was involved in epithelial-mesenchymal transition (EMT) induced by high glucose in A20 murine diffuse large B-cell lymphoma (DLBCL) cells by regulating the Wnt/b-catenin signaling pathway. A20 cells were transfected with siHMGA2 (50 nM), and then treated with high glucose (30 mM) for 48 hours. Western blot was performed to explore the effects of HMGA2 knockdown on the Wnt/b-catenin signaling pathway. The expression of key proteins including b-catenin, cyclin D1 and c-myc in the signaling pathway was assessed, and quantification analysis was performed and shown as a graph right next to the images. GAPDH was used as an internal reference. Each experiment was performed in triplicate and representative images are shown. Data are presented as the mean ± standard deviation (SD). * P<0.05, compared with the Control (normal glucose) group.

    Article Snippet: A20 murine diffuse large B-cell lymphoma (DLBCL) cells (ATCC, Manassas, VA, USA) were routinely cultured in complete RPMI1640 medium (Gibco, Thermofisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS), and maintained at 37°C in a humidified atmosphere containing 5% CO2.

    Techniques: Expressing, Transfection, Western Blot, Knockdown, Standard Deviation, Control

    Figure 6. Graphic conclusion. High glucose upregulates HMGA2 to regulate the Wnt/b-catenin signaling pathway and induces epithelial-mesenchymal transition (EMT), further promoting invasion and metastasis in diffuse large B-cell lymphoma (DLBCL).

    Journal: Medical Science Monitor

    Article Title: High Glucose Promotes Epithelial-Mesenchymal Transition, Migration and Invasion in A20 Murine Diffuse Large B-Cell Lymphoma Cells Through Increased Expression of High Mobility Group AT-Hook 2 (HMGA2)

    doi: 10.12659/msm.916195

    Figure Lengend Snippet: Figure 6. Graphic conclusion. High glucose upregulates HMGA2 to regulate the Wnt/b-catenin signaling pathway and induces epithelial-mesenchymal transition (EMT), further promoting invasion and metastasis in diffuse large B-cell lymphoma (DLBCL).

    Article Snippet: A20 murine diffuse large B-cell lymphoma (DLBCL) cells (ATCC, Manassas, VA, USA) were routinely cultured in complete RPMI1640 medium (Gibco, Thermofisher Scientific, Waltham, MA, USA) supplemented with 10% fetal bovine serum (FBS), and maintained at 37°C in a humidified atmosphere containing 5% CO2.

    Techniques: